Table 3.

qRT-PCR-based expression profiles of clpB

Total RNA was isolated from mid-exponential phase cultures that were kept at 37 °C (control) or incubated at 48 °C for 30 min. The results were obtained using qRT-PCR as described in Methods. Data are means±sd of three independent experiments performed with RNA isolated from three independent cultures. All samples were assayed in triplicate. nd, Not done.

Strain	Relative clpB mRNA (copies μl−1)
OG1RF (37 °C)	8.53×104±1.33×104
OG1RF (48 °C)	1.14×106±4.41×105*
ΔctsR (37 °C)	4.37×106±1.99×106
ΔctsR (48 °C)	1.00×107±nd*

*Significant difference compared with the control (P≤0.05).