Figure 5.

Extended E^rns substrate specificity. Various dilutions of Strep-tag purified wild-type (C171), monomeric (R171) and RNase-inactive mutant (H30F) E^rns were incubated with 625 nM of poly(U), poly(A), poly(C) and poly(G), either labeled in green or red (panels a–d) as described in Table 1. Samples were separated by 14% PAGE and fluorescence was analysed with an Azure c600 imaging system. Due to the known, defined length of the directly labeled fragments, no size ladder was applied. Non-cropped gels as representative experiment out of three are shown.