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. 2018 May 29;8:8259. doi: 10.1038/s41598-018-25646-8

Figure 5.

Figure 5

FcRγ-coupled LMIR8 is a suitable marker for PDC. (a) BMMCs transduced with Flag-tagged LMIR8 or mock were stimulated with plate-coated anti-LMIR8 Ab or a control Ab or with 100 nM PMA or PBS as a control. IL-6 released into the culture supernatants were measured by ELISA. All data points correspond to the mean ± S.D. of three independent experiments. Statistically significant differences are shown. *p < 0.01 (Student’s t-test). (b) BM cells from WT or FcRγ−/− mice were stained with FITC-conjugated anti-CD11c Ab and biotinylated anti-LMIR8 Ab or biotinylated rat IgG2a Ab followed by PE-conjugated streptavidin. (c) BM cells from WT mice before or 24 h after an intravenous injection of 12.5 μg of CpG ODN D19 were stained with either FITC-conjugated anti-Siglec-H Ab, anti-CD19 Ab, or anti-CD11b Ab and biotinylated anti-LMIR8 Ab or biotinylated rat IgG2a Ab followed by PE-conjugated streptavidin. Siglec-H+ (left panel), CD19+ (middle panel), or CD11b+ (right panel) cell populations were gated and analyzed for LMIR8 expression. One representative of four independent experiments is shown.