Figure 6.

LMIR8 engagement with anti-LMIR8 Ab inhibits the TLR9-mediated cytokine production in transduced pDCs. (a) WT BMpDCs transduced with Flag-tagged LMIR8 or mock were stained with FITC-conjugated anti-CD11c Ab and APC-conjugated anti-B220 Ab or with biotinylated anti-LMIR8 Ab or biotinylated rat IgG2a Ab followed by PE-conjugated streptavidin. One representative of three independent experiments is shown. (b,c) Flag-tagged LMIR8-transduced WT BMpDCs were stimulated with plate-coated anti-LMIR8 Ab or a control Ab (b) or with anti-Flag Ab or a control Ab (c) in the presence or absence of 5 μM CpG ODN 1585 or PBS as a control. IFN-α released into the culture supernatants were measured by ELISA. All data points correspond to the mean ± S.D. of three independent experiments. Statistically significant differences are shown. *p < 0.01 (Student’s t-test).