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. 2018 May 29;8:8234. doi: 10.1038/s41598-018-26481-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of CHM3 iPSC and iPSC-derived RPE. qPCR analysis shows the mRNA levels of the host pluripotency markers OCT3/4 (A), LIN28 (B), SOX2 (C) and NANOG (D) in CHM3 iPSC (black bars) as compared to the original fibroblasts. Data are expressed as mean ± SEM, n = 3. (E) Karyotype analysis of the CHM3 iPSC did not detect large chromosomal rearrangements. (F) The pigmented, cobblestoned appearance of the iPSC-derived RPE monolayer. (G) qPCR analysis of the expression of the typical RPE markers MERTK, RDH5, TYR, ZO-1, PAX6, BEST1 and RLBP1 shows an upregulation of expression (in relative units) in the CHM3 iPSC-derived RPE (grey bars) as compared to fibroblasts (black bars). Data are expressed as mean ± SEM, n = 3. (H) Weekly recordings of the transepithelial resistance (TER) following P3 seeding are presented as normalized Ω/cm². Data are expressed as mean ± SEM, n = 3.