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. 2018 May 29;8:8218. doi: 10.1038/s41598-018-26425-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of ShT-N and ShT-P colonies. (A) A mixture of pigmented (ShT-P) and non-pigmented (ShT-N) colonies of ATCC 33728^T on 7H11 solid medium. (B) Subcloned colonies of ATCC 33728^T. ShT-P and ShT-N were isolated and propagated. Only pigmented, yellow colonies were produced by ShT-P, whereas for ShT-N only non-pigmented, ivory colonies reproduced. The mycolactone-producing “M. shinshuense” strain Sh-753 was used as a positive control.