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. 2018 May 29;8:8218. doi: 10.1038/s41598-018-26425-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silica thin-layer chromatography and ESI/MS/MS analyses of acetone soluble lipids (ASLs) from M. ulcerans or “M. shinshuense” isolates. (A) ASLs extracted from the bacterial cell mass (CM). Samples were loaded as follows: lane 1, MU-1615 (M. ulcerans Malaysian strain, virulent); lane 2, Sh-753 (“M. shinshuense”, virulent); lane 3, ShT-N (ATCC 33728, non-pigmented colony); lane 4, ShT-P (ATCC 33728, pigmented colony); lane 5, MU-4 (M. ulcerans 97–107 African strain, virulent); lane 6, MU-8; (M. ulcerans 5143 Mexican strain, avirulent). Arrows show the position of mycolactone and phthiocerol (control lipid). (B) The ESI/MS spectrum of ASLs from the strain ShT-P detected the peaks of m/z 765.4, 763.4, 779.4 as [M + Na]⁺. In each MS/MS spectrum, the distinct ions were found m/z 359 and 429 for mycolactone A/B; m/z 357, 429 and 705 for mycolactone S1; m/z 373, 429 and 705 for mycolactone S2, respectively. These molecular and fragment ions were fixed the proposed structures of each mycolactone.