Table 1.
Characteristics of bacteria tested in this study.
| “M. shinshuense” | M. ulcerans | ||||
|---|---|---|---|---|---|
| ATCC 33728 | MU-4 | MU-8 | |||
| ShT-P (pigmented) | ShT-N (non-pigmented) | Sh-753 | |||
| Mycolactone production | + | − | + | + | − |
| Colony: | |||||
| Color (pigmentation) | + | − | + | + | − |
| Morphology | R | R | R | R | R |
| Growth on 2% Ogawa medium slant | |||||
| 14 days | + | + | + | − | − |
| 30 days | + | + | + | + | + |
| Growth at: | |||||
| 25 °C | + | + | + | + | + |
| 32 °C | + | + | + | + | + |
| 37 °C | − | − | − | − | − |
| 42 °C | − | − | − | − | − |
| Growth on 500 μg/ml p-nitrobenzoic acid | − | − | − | − | − |
| Growth on NaCl medium (5%) | − | − | − | − | − |
| Tween 80 hydrolysis | − | − | − | − | − |
| Nitrate reduction | − | − | − | − | − |
| Arylsulfatase | − | − | − | − | − |
| Pyrazinamidase | − | − | − | − | − |
| Iron uptake | − | − | − | − | − |
| MPB64 Ag production | − | − | − | − | − |
| Semi−quantitative catalase | + | + | + | + | + |
| 68 °C catalase | + | + | + | + | + |
| Urease | + | + | + | − | − |
R: rough. See Fig. 2 for visualization of mycolactone production by TLC.