Table 2.
PCR detection of eight giant plasmid-associated genes in M. ulcerans or “M. shinshuense” strains.
| pMUM001 marker genes | ||||||||
|---|---|---|---|---|---|---|---|---|
| repA | parA | MUP011 (STPK) | mls | mlsAT | MUP038 (TEII) | MUP045 (KSIII) | MUP053 (p450) | |
| ShT-P | + | + | − | + | + | + | + | + |
| ShT-N | − | − | − | − | − | − | − | − |
| Sh-753 | + | + | − | + | + | + | + | + |
| MU-4 | + | + | + | + | + | + | + | + |
| MU-1615 | + | + | + | + | + | + | + | + |
| MU-8 | + | + | − | + | − | − | + | + |
ShT-P: ATCC 33728, pigmented strain; ShT-N: ATCC 33728, non-pigmented strain; Sh-753: “M. shinshuense”, virulent; MU-4: M. ulcerans 97–107 African strain, virulent; MU-1615: M. ulcerans Malaysian strain, virulent; MU-8: M. ulcerans 5143 Mexican strain, avirulent; STPK, serine/threonine protein kinase gene; mlsAT, acyltransferase domain of mls; TEII, type II thioesterase gene; KSIII, type III ketosynthase gene; p450, p450 hydroxylase gene.