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. 2018 May 29;8:8318. doi: 10.1038/s41598-018-26777-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NS4B co-localizes with FHC. PK-15 cells were co-transfected with pEGFP-NS4B and pFHC-Red as well as pEGFP-C1and pDsRed-N1. Cells were fixed at 48 hpt and nuclei were visualized by staining with DAPI. The GFP-NS4B fusion protein was shown to co-localize with the FHC-Red protein in both CSFV-infected and -uninfected cells.