Table 1.
Primers in this research.
| primers | sequence(5′−3′) | purpose |
|---|---|---|
| GST-FHC-F | CGGGATCC ATGACGACCTCGTGCTCCTC | Amplification of GST-FHC |
| GST-FHC-R | GGAATTCTTA GCTCTCACTGCTCCCCAGG | |
| GFP-FHC-F | CGGAATTCTATGACGACCTCGTGCTCCTCG | Amplification of GFP-FHC |
| GFP-FHC-R | CGGGATCCTTAGCTCTCACTGCTCCCCAGGGTG | |
| FHC-Red-F | GGAATTC ATGACGACCTCGTGCTCCTC | Amplification of FHC-Red |
| FHC-Red-R | CGGGATCCCGGCTCTCACTGCTCCCCAGG | |
| Flag-FHC-F | GGAATTCCATGGATTACAAGGATGACGACGATAAGATGACGACCTCGTGCTCCTC | Amplification of Flag-FHC |
| Flag-FHC-R | CGGGATCCCGCTCTCACTGCTCCCCAGG | |
| GST-NS4B-F | CGGGATCCTATGGCTCAGGGGGATGTGC | Amplification of GST-NS4B |
| GST-NS4B-R | GGAATTCTTATAGCTGGCGGATCTTTCC | |
| GFP-NS4B-F | GGAATTCTATGGCTCAGGGGGATGTGC | Amplification of GFP-NS4B |
| GFP-NS4B-R | CGGGATCCTTATAGCTGGCGGATCTTTCC | |
| FHC-sh1-F | GATCCGCAGGTGAAAGCCATCAAAGACAAGAGTCTTTGATGGCTTTCACCTGCTTTTTG | knockdown of FHC |
| FHC-sh1-R | AATTCAAAAAGCAGGTGAAAGCCATCAAAGACTCTTGTCTTTGATGGCTTTCACCTGCG | |
| FHC-sh2-F | GATCCGCTGGAACTGCACAAACTGGCCAAGAGGCCAGTTTGTGCAGTTCCAGCTTTTTG | knockdown of FHC |
| FHC-sh2-R | AATTCAAAAAGCTGGAACTGCACAAACTGGCCTCTTGGCCAGTTTGTGCAGTTCCAGCG | |
| FHC-sh3-F | GATCCGGATATCATGAAACCGGAGCGCAAGAGCGCTCCGGTTTCATGATATCCTTTTTG | knockdown of FHC |
| FHC-sh3-R | AATTCAAAAAGGATATCATGAAACCGGAGCGCTCTTGCGCTCCGGTTTCATGATATCCG | |
| shN-F | GATCCGCTTAAACGCATAGTAGGACTTCAAGAGAGTCCTACTATGCGTTTAAGCTTTTTG | negative control of knockdown |
| shN-R | AATTCAAAAAGCTTAAACGCATAGTAGGACTCTCTTGAAGTCCTACTATGCGTTTAAGCG | |
| FHC-F | CCGCGATGATGTGGCTTTG | qPCR for detection of FHC |
| FHC-R | GGTTTCATGATATCCTGAAGG | |
| β-actin-F | CAAGGACCTCTACGCCAACAC | qPCR for detection of β-actin |
| β-actin-R | TGGAGGCGCGATGATCTT | |
| CSFV-F | GATCCTCATACTGCCCACTTAC | qPCR for detection of CSFV |
| CSFV-R | GTATACCCCTTCACCAGCTTG |
Underline show restriction enzyme sites or loop ring, bolds show Flag tag.