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. 2018 May 29;8:8196. doi: 10.1038/s41598-018-26399-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relevant substrates, intermediates, products, and enzyme kinetic parameters for (A) native Pdu microcompartment metabolism and (B) mevalonate biosynthesis. Predicted pathway flux for (C) native Pdu microcompartment metabolism and (D) mevalonate biosynthesis for native kinetics without organization; 100-fold improvement of k_cat for both pathway enzymes; 100-fold improvement in K_M for both pathway enzymes; native kinetics with organization on a scaffold; and native kinetics with organization in a microcompartment organelle. The predictions here are based on an external substrate concentration of 50 mM 1,2-propanediol, as is typically used in experiments⁴². We use the same external substrate concentration (50 mM) in the mevalonate case. Experimental observations in (C) and (D) are calculated from S. enterica⁴² and from titer measurements for a scaffolded system from⁶³.