Fig. 2.

Localization of FPN with respect to SCV-associated marker proteins. a HeLa cells transfected with pFPN-EGFP were stained with an anti-FPN antibody and visualized by confocal microscopy. Scale bar, 10 μm. b Live cell images after treatment of HeLa cells expressing FPN-GFP with hepcidin. Arrows indicate FPN on the plasma membrane. Scale bar, 50 μm. c HeLa cells expressing FPN-GFP were infected with Salmonella (MOI, 100) and stained for the markers of early and late SCV (EEA1 and Rab5 at 1 h p.i. and LAMP1 at 12 h p.i.). Scale bar 10 μm. Images are representatives of over 100 infected cells from at least six independent experiments. The images on the left were taken at ×1000 magnification and on the right at ×2000 magnification, which were marked in dotted box on the left panel