Fig. 5. MPT0G211 significantly decreased the phosphorylation of tau by GSK3β inactivation.

a, b SH-SY5Y and Neuro-2a cells were transfected for 24 h with pCAX APP 695 and pRK5-EGFP-Tau P301L and incubated with MPT0G211 or ACY-1215 (0.1 μM) for another 24 h, after which the cell lysates were subjected to immunoblotting. c SH-SY5Y cells were incubated with or without Aβ_1–40 (10 μM) for 24 h and then with MPT0G211 (0.1 μM) for a further 24 h. Cell lysates were prepared for western blot analysis of the indicated proteins. Results are presented as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared with the indicated groups