Table 1.
Model parameters for two cell conditions in CHO-K1 cell line.
Model Parameters | Cell Condition | |
---|---|---|
Plateau Phase | Logarithmic Growth Phase | |
α1 = A1〈G1〉(=α0) (Gy−1) | 0.155 ± 0.027 | 0.075 ± 0.025 |
β1 = B1〈G12〉 (=β0) (Gy−2) | 0.048 ± 0.003 | 0.028 ± 0.008 |
0.704 ± 0.118 | 1.782 ± 0.441 | |
Relative 〈G1〉 (=〈G〉) | 1.000 ± 0.003 | 1.227 ± 0.009 |
Relative 〈G12〉 (=〈G2〉) | 1.000 ± 0.007 | 1.457 ± 0.050 |
ρ (g · cm−3) | 1.000 (water) | 1.000 (water) |
rd (μm) | 0.500 | 0.500 |
0.924 (250 kVp) | 0.924 (250 kVp) | |
Each Phase | Cell Cycle distribution [%] | |
Plateau Phase | Logarithmic Growth Phase | |
G0/G1 | 72.7 ± 6.6 | 37.6 ± 3.8 |
S | 14.9 ± 2.3 | 52.4 ± 4.5 |
G2/M | 12.4 ± 3.9 | 11.4 ± 0.7 |
The set of values for plateau phase was obtained from our previous report12. In these parameters, a is a few percentage of c17,20. Relative mean DNA amount per nucleus was normalized by that for plateau phase. The set of parameters for logarithmic growth phase was converted from that for plateau phase (SD was deduced by error propagation). In the parameters, (a + c) value for logarithmic phase was deduced from an experimental cell recovery data33, and DNA amount represented by 〈G〉 and 〈G2〉 was obtained within the performed cell-cycle experiment. The set of physical parameters (γ, yD, rd, ρ) was taken from the previous reports12.