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. 2018 May 29;38(12):e00102-18. doi: 10.1128/MCB.00102-18

FIG 3.

FIG 3

PTPL1 binds PTEN and ensures apical enrichment of PTEN. (A) Localization of GFP-PTPL1 and Lifeact-Ruby in polarized W4 cells. Scale bar, 5 μm. (B) Coimmunoprecipitation (IP) of HA-PTPL1 with YFP-PTEN wt and ΔPBM in HEK293T cells. TL, total lysate. (C) Localization of YFP-PTEN wt and ΔPBM in W4:shCTRL cells and of YFP-PTEN wt in PTPL1-depleted cells in combination with Lifeact-Ruby. Scale bars, 5 μm. (D) Quantification of apical enrichment of YFP-PTEN wt and ΔPBM in control or PTPL1-depleted W4 cells. Red bars represent the average, and error bars represent the SEM (n > 10). *, P < 0.05 using independent sample t tests.