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. 2018 May 29;38(12):e00102-18. doi: 10.1128/MCB.00102-18

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Copyright © 2018 Bruurs et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — Deletion of PTEN in mouse small intestinal organoids does not result in severe morphological defects. (A) Uninduced (PTEN^+/+) and induced (PTEN^−/−) mouse small intestinal organoids stained for actin (phalloidin-Alexa Fluor 568) and DNA (DAPI). Scale bars, 50 μm. Asterisks indicate the position of the central lumen. (B) Ratio of apical versus basal membrane length of enterocytes that line the central lumen. Red bars represent the average. Error bars represent the standard deviation (SD) (n > 11 organoids in at least two experiments). n.s., not significant using independent sample t tests (P > 0.05). (C) Western blot of uninduced and induced organoid lysates probed for PTEN and GAPDH.