FIG 2.

S396 phosphorylation and R282 cooperate to promote TIN2L binding to TRF2. (A) Representative co-IP of TRF2 with cotransfected wild-type TIN2S/L, TIN2S/L-R282H, or empty vector (EV). (B) Representative co-IP of TRF2 with cotransfected wild-type TIN2S/L, phosphomimetic TIN2L (TIN2L-S396E), phosphodead TIN2L (TIN2L-S396A), or EV. (C) Representative co-IP of TRF2 with cotransfected wild-type TIN2S/L, TIN2L-R282H, TIN2L-S396A, double mutant TIN2L-R282H+S396A, or EV. (D) Quantification of data in panels A, B, and C. For quantification, the amount of TRF2 coimmunoprecipitated was divided by the amount of TIN2 immunoprecipitated in order to account for any differences in TIN2 expression/pulldown. For each experiment, the value was then normalized to that of TIN2S. Error bars represent the SDs of several separate co-IP experiments. *, P < 0.008.