FIG 6.

Endogenous TIN2L cofractionates with other shelterin components in HeLa cell nuclear extract. (A) Western blot analysis of shelterin components from size-based fractionation of HeLa cell nuclear lysate. HeLa cell nuclear extracts were subjected to gel filtration, and 0.5-ml fractions were taken beginning at a 30-ml elution volume. Aliquots of 50 μl were taken from the indicated fractions and analyzed for the presence of TIN2, TRF1, TRF2, and POT1 by immunoblotting. The molecular size indicated was determined using a molecular size standard. (B) Quantification of data in panel A. Values were normalized to the fraction with the largest amount of each respective protein.