FIG 2.

RBM4-associated brain transcripts include Dab1. (A) Flow diagram for the strategy used to identify RBM4-associated transcripts in embryonic mouse brain. Two independent immunoprecipitation experiments of E13.5 brain lysates were performed using control (IgA) or anti-RBM4. Immunoprecipitated RNA was subjected to RNA-Seq. A total of 68 genes were common between the top 500 of each individual batch obtained through similar transcripts and event identifications (ID) (TSS represents alternative 5′ first exon and transcription start site). (B) Gene ontology analysis of the aforementioned 68 RBM4-associated transcripts with respect to molecular function categories. (C) A list of 11 potential RBM4 targets that are involved in neuronal migration. (D) The graph shows the top 500 genes plotted by the ratio of RBM4/IgA (log₁₀) versus rank in two experiments. Dab1 is indicated. (E) As in panel A, each E13.5 brain cell lysate was subjected to immunoprecipitation (IP) using anti-RBM4 or anti-IgA. RT-PCR and immunoblotting (IB) were performed to detect the indicated RNAs and proteins. Lane 1, 2% input.