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. 2018 May 29;38(12):e00007-18. doi: 10.1128/MCB.00007-18

FIG 6.

FIG 6

RBM4 modulates neuronal migration via Dab1. (A) The scheme illustrates the CAG-Dab1-mNES-DsRed and CAG-Dab1Δ78-mNES-DsRed vectors. mNES represents mutated NES. (B) The vectors shown in panel A were each transfected into HEK293 cells; immunoblotting was performed with antibodies against Dab1 and GAPDH. (C and D) IUE was performed as described in the legend to Fig. 1. In brief, for panel C, each wild-type E13.5 brain was injected with the GFP-containing control or shRBM4 expression vector alone or together with the Dab1-mNES or Dab1Δ78-mNES expression vector. For panel D, each Rbm4a KO E13.5 brain was injected with a dsRED-containing control or Dab1-mNES-DsRed expression vector (scale bar, 100 μm). In representative images, Hoechst stain (blue) indicates cell nuclei, and EGFP (green) and DsRed (Red) represent the expression of shRNA and recombinant Dab1, respectively. The bar graphs in panels C and D are presented as described in the legend to Fig. 1H. *, P < 0.05; **, P < 0.01; ***, P < 0.001.