FIG 3.

HCMV viral protein expression and cVAC formation are unaffected by dominant negative ESCRT-III subunits. (A) Western blot analysis of viral proteins 96 hpi (MOI, 3) in fibroblasts expressing CHMP6-GFP, CHMP4B-GFP, or CHMP4C-GFP. Hours (above the blot) indicate the duration of Dox induction. (B) Immunofluorescence staining for pp28 (red) and gB (green) 96 hpi. Fibroblasts were treated with Dox for 48 h prior to fixation. Nuclei were labeled with DAPI. Bars, 10 μm. (C to E) Cell viability assays on fibroblasts expressing GFP, CHMP6-GFP, CHMP4B-GFP, or CHMP4C-GFP 96 hpi (MOI, 3) as measured by a trypan blue (C), XTT (D), or CellTiter-Glo (E) assay. For each cell type, viability is shown relative to no Dox treatment. Significant differences (P < 0.05) between samples are marked with asterisks. All viability assay results are from at least three independent experiments.