FIG 5.

Dominant negative ESCRT-III does not inhibit HCMV secondary envelopment. (A) Electron micrographs of enveloped intracellular and extracellular virions in fibroblasts expressing GFP, CHMP6-GFP, or CHMP4B-GFP at 96 h after infection with AD169 (MOI, 3). Fibroblasts were treated with Dox for 48 h prior to fixation. (B) Graph showing percentages of membrane-associated capsids that have not completed envelopment compared to percentages of fully enveloped capsids. Total numbers of capsids were 182 (GFP), 190 (CHMP6), and 181 (CHMP4B).