FIG 5.

Latency clearance assay. (A) Resting CD4⁺ T cells were incubated with PHA or VOR for 24 h, cocultured with or without autologous NK cells at an E:T ratio of 1:10 for another 24 h, and plated in 12 replicates in the presence of feeders for 19 days, refreshing the medium every 3 or 4 days. The supernatant was assayed for p24 ELISA to assess the presence of HIV replication. The graphs show the numbers of wells in which p24 was detected at day 19, with a different color for each donor. (Left) Reactivation with PHA (n = 6). (Right) Reactivation with 335 nM VOR (n = 8). Wilcoxon matched-pairs signed-rank test. (B) Latency clearance assays in which NK cells were depleted after the initial 24 h of coculture with VOR-reactivated CD4⁺ T cells (n = 5). IL-15-stimulated NK cells reduced the number of HIV⁺ wells during the first 24 h of coculture with VOR-reactivated CD4⁺ T cells. The error bars indicate SEM.