Degradation of Tab182 following infection with adenovirus serotype 5 or adenovirus serotype 12 is dependent on the adenovirus E1B55K protein. (A and B) HeLa cells were infected with adenovirus serotype 5 (A) or serotype 12 (B) at 5 PFU/cell. (C and D) HeLa cells were also infected with the adenovirus serotype 5 E1B55K-negative virus Ad5dl1520 (C) and the adenovirus serotype 12 E1B55K-negative virus Ad12dl620 (D) at 10 PFU/cell. Cells were then harvested at various time points (0, 8, 24, 48, 72, and 96 h) postinfection. Cell lysates were subjected to SDS-PAGE and Western blotting using the indicated antibodies.