Expression of cyclin E and is enhanced in Tab182- and CNOT1-depleted cells. HeLa cells were transfected with control, Tab182, or CNOT1 siRNAs. Forty-eight hours later, control, Tab182, and CNOT1 siRNA-treated cells were mock infected (A) or infected with adenovirus serotype 12 (B) at 5 PFU/cell. Cells were then harvested at various time points (0, 8, 24, 48, 72, and 96 h) postinfection. Cell lysates were subjected to SDS-PAGE and Western blotting using the indicated antibodies.