FIG 13.

The relative expression level of Ad13S E1A mRNA is increased in infected cells in the absence of CNOT1 or Tab182. HeLa cells were transfected with control, Tab182, or CNOT1 siRNAs, and 48 h later, they were infected with Ad5 (A) or Ad12 (B) at 5 PFU/cell. Cellular RNA was extracted from infected cells, and first-strand cDNA synthesis was carried out. RT-PCRs were performed by using Ad13SE1A CR3 region-specific primers and real-time PowerUp SYBR green master mix. To check E1A relative gene expression levels, calculated E1A C_T values were normalized to C_T values of GAPDH amplified from the same sample [ΔC_T = C_T (E1A) − C_T (GAPDH)], and the 2^−ΔΔC_Tmethod was used to calculate relative gene expression levels. Data are the means of results of 3 repeats. Statistical significance was determined by using Student's t test, and P values of less than 0.05 (*) or 0.01 (**) were considered significant. Error bars represent standard errors of the means.