FIG 14.
Viral DNA synthesis is increased in Tab182- and CNOT1-depleted cells after adenovirus infection. HeLa cells were treated with control, Tab182, and CNOT1 siRNAs for 48 h and then infected with Ad5 (A) or Ad12 (B) at 5 PFU/cell. After 24 h, cells were harvested, and the total DNA was isolated. Quantitative PCR was performed to determine the relative concentration of viral DNA. Hexon CT values were normalized to CT values for GAPDH DNA amplified from the same sample. Data are the means of results from 3 repeats. Statistical significance was determined by using Student's t test, and P values of less than 0.05 (*) or 0.01 (**) were considered significant. Error bars represent standard errors of the means.