FIG 2.

Degradation of Tab182 following infection with adenovirus serotype 5 is dependent on the adenovirus E4orf6 protein. HeLa cells were infected with the Ad5 E4 mutants H5in351 (E4orf1⁻) (A), H5pm4154 (E4orf6⁻) (A), H5pm4155 (E4orf3⁻ E4orf6⁻) (B), H5pm4166 (E4orf4⁻) (B), H5dl356 (E4orf6⁻ E4orf7⁻) (C), H5in352 (E4orf2⁻) (C), and H5pm4150 (E4orf3⁻) (D) at 10 PFU/cell. Cells were then harvested at various time points (0, 8, 24, 48, 72, and 96 h) postinfection. Cell lysates were subjected to SDS-PAGE and Western blotting using the indicated antibodies.