FIG 3.
Tab182 gene expression is enhanced in adenovirus-infected cells. HeLa cells were infected with Ad5 or Ad12 at 5 PFU/cell. Cells were harvested at various time points (0, 8, 24, 48, 72, and 96 h) postinfection. Cellular RNA was extracted from Ad5 (A)- and Ad12 (B)-infected cells, and first-strand cDNA synthesis was carried out. RT-PCRs were performed by using Tab182-specific primers and real-time PowerUp SYBR green master mix. To determine the relative Tab182 gene expression level, calculated Tab182 CT values were normalized to CT values of GAPDH amplified from the same sample [ΔCT = CT (Tab182) − CT (GAPDH)], and the 2−ΔΔCT method was used to calculate relative expression levels. Each experiment was performed in triplicate. Western blots of Ad5- and Ad12-infected HeLa cells were performed to confirm Tab182 degradation (data not shown).