FIG 8.

Tab182 does not localize to viral replication centers during adenovirus infection. GFP-Tab182 was transfected into HeLa cells, and 24 h later, cells were infected with Ad5 or Ad12. (A) Thirty hours later, cells were fixed, extracted, and probed with the appropriate antibodies. (B) Thirty hours after infection, cells were preextracted as described in Materials and Methods before fixing and then staining with antibodies. In both panels A and B, Ad5-infected cells were probed with DBP antibody, while Ad12-infected cells were probed with RPA32 antibody. Nuclear DNA is stained with DAPI.