TABLE 2.
Primers used in this study
| Gene | Sequence (5′→3′) | Length (bp) | Start position | Stop position | PCR product size (no. of bases) |
|---|---|---|---|---|---|
| E1A-Ad5 (CR3) | Forward, TAGATTATGTGGAGCACCCCG | 21 | 990 | 1010 | 110 |
| Reverse, GCCACAGGTCCTCATATAGCAA | 22 | 1099 | 1078 | ||
| E1A-Ad12 (CR3) | Forward, AGTCCTGTGAGCACCACCG | 19 | 980 | 1053 | 74 |
| Reverse, GTAGGCTCGCAGATAGCACA | 20 | 998 | 1034 | ||
| Tab182 | Forward, CTGCTCTGAGGGACTCCTTG | 20 | 2310 | 2329 | 158 |
| Reverse, CTGGGTCTCCTCTAGGGCTT | 20 | 2448 | 2467 | ||
| GAPDH (RNA) | Forward, GAGTCAACGGATTTGGTCGT | 20 | 53 | 72 | 183 |
| Reverse, ACAAGCTTCCCGTTCTCAG | 19 | 218 | 236 | ||
| GAPDH (DNA) | Forward, CGGCTACTAGCGGTTTTACG | 20 | 6534369 | 6534388 | 188 |
| Reverse, AGAAGATGCGGCTGACTGT | 20 | 6534538 | 6534557 | ||
| Hexon (Ad5) | Forward, GCCACGGTGGGGTTTCTAAACTT | 23 | 18862 | 18882 | 127 |
| Reverse, GCCCCAGTGGTCTTACATGCACATC | 25 | 18967 | 18989 | ||
| Hexon (Ad12) | Forward, GCCACGGTGGGGTTTCTAAACTT | 23 | 17764 | 17784 | 127 |
| Reverse, GCCCCAGTGGTCTTACATGCACATC | 25 | 17869 | 17891 |