FIG 6.
Validation of HA-tagged VZV ORF63-expressing HaCaT cells. (A) HaCaT cells (9 × 105) were transduced with VZV ORF63 or CT pseudoviruses and were selected with 0.5 mg/ml G418 for 10 days to create VZV ORF63, CT, and untransduced HaCaT cells. VZV ORF63, control transduced, and untransduced HaCaT cells (1 × 105) were seeded on coverslips (13 mm; Knittel glass), fixed with 4% PFA, and stained for HA (red) and VZV ORF63 (green). The cells were counterstained with nuclear DAPI (blue) and visualized by fluorescence microscopy. The images are shown at ×20 magnification. (B) Additionally, 5 × 105 VZV ORF63, CT, and UT HaCaT cells were fixed, permeabilized, stained for HA, and analyzed via flow cytometry. All the data presented are representative of three biological replicates.