FIG 5.
Construction of cysteine and leucine zipper mutant recombinant viruses. (A) Prediction of GP41 oligomerization motifs. The leucine zipper-like coiled-coil domain was predicted by PCOILS and is presented by use of helical wheel projection software. The key leucine residues are presented as green diamonds. (B) Construction of cysteine and leucine zipper-like motif mutant recombinant viruses. The cysteines and key leucines were mutated individually or in combination by site-directed mutagenesis. The ph gene and each of the mutant gp41 genes were coinserted into the ph locus of the AcΔgp41 bacmid to construct AcΔgp41-gp41x-ph.