FIG 8.

Oligomerization and subcellular localization of leucine zipper-like motif mutants. (A) Oligomerization of leucine zipper-like motif mutant GP41. Sf9 cells were infected with single-site mutants or transfected with double-site mutants and collected at 36 h p.i. or 48 h p.t. After being treated under nonreducing (NR) or reducing (R) conditions, cell samples were analyzed by Western blotting using anti-GP41 pAb. GAPDH was detected as a control. (B) Subcellular localization of leucine zipper-like motif mutants. Cells were infected or transfected with leucine zipper-like motif mutants and fixed at 36 h p.i. or 48 h p.t. The locations of GP41 mutants were detected by immunofluorescence microscopy with anti-GP41 pAb. The enlarged fields show the boxed regions of the TRITC and DAPI channels. Bars, 5 μm for the enlarged images and 10 μm for the others.