FIG 2.

Dynein is not required for SV40-induced focus formation. (A) Representative images of CV-1 cells infected with SV40 (MOI, ∼20) that were treated with either the control (DMSO) or 100 μm cilioD (at 5 hpi). After 16 h, cells were fixed and were immunostained with the indicated antibodies. (B) Quantification of the results shown in panel A. Cells harboring at least 1 BAP31-positive focus were scored as positive, and at least 100 cells were scored for each experiment. The data are normalized to those for the DMSO control (shaded bar). Values are averages of the means (n = 3) ± SD. (C) Quantification of focus formation in SV40-infected CV-1 cells expressing the indicated construct. The data are normalized to those for the GFP control (shaded bar). Values are averages of the means (n = 3) ± SD.