FIG 1.

Effect of SPCS1 knockdown on propagation of JEV. (A) HEK-293 cells were transfected with three different siRNAs targeted against SPCS1, or a control siRNA, at a final concentration of 15 nM. At 48 hpi, cells were infected with JEV at an MOI of 0.5. Two days after infection, JEV antigen-positive cells were identified by indirect immunofluorescence assays using JEV E protein-specific monoclonal antibodies. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Cell infectivity was examined by using an HCS system. The results are the averages of data from three independent experiments performed in triplicate. (B) Cell viability following siRNA transfection, determined by using 3-(4,5-dimethylthiazol-2-yl)-(2,5-diphenyltetrazolium bromide)-tetrazolium (MTT) cell viability assays. The data are pooled from three experiments in duplicate. Statistical significance was determined by analysis of variance with a multiple-comparison correction (**, P < 0.01; ***, P < 0.001).