FIG 3.

Effects of JEV propagation in SPCS1 KO HEK-293 cells following complementation with a plasmid expressing SPCS1. (A) WT HEK-293 cells, SPCS1 KO HEK-293 cells, and SPCS1 KO HEK-293 cells cotransfected with a plasmid expressing SPCS1 (or a control plasmid) were infected with JEV at an MOI of 0.5. At 48 hpi, cells were fixed and probed with E protein-specific monoclonal antibodies by an IFA. Data from one experiment of three are shown. (B) Infectivity analyzed by using the HCS system. The data are pooled from three experiments in duplicate. Statistical significance was determined by Student's t test (****, P < 0.0001). (C) Cell cytopathic effects in infected cells observed at 72 hpi with the crystal violet staining method. Data from one experiment of three are shown.