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. 2017 Aug;187(8):1787–1799. doi: 10.1016/j.ajpath.2017.04.013

Figure 1.

Figure 1

Modeling esophageal inflammation. A: Illustration of the standard OTC method and the modification introduced by incorporating PBMCs into the collagen matrix to model inflammation. B: Representative images show H&E staining of normal human esophageal keratinocytes grown under standard OTC conditions. C: CD45 IHC staining of an OTC with human PBMCs and IL-2 added to maintain PBMC viability. CD45+ staining indicates the presence of immune cells in the collagen matrix. Inset: Higher power magnification of CD45+ cells. D: IHC staining for the cell proliferation marker Ki-67 in an OTC+PBMC culture with only IL-2 to maintain PBMC viability. Ki-67+ cells noted in the epithelium (brown arrows) but not in stromal cells. Inset: Higher power magnification of Ki-67− stromal cells. E: IHC staining for the cell proliferation marker Ki-67 in an OTC+PBMC+IL culture (IL-2, -7, and -15 included) to induce an acute inflammatory response. Ki-67+ cells noted in both the epithelium and stromal cells (brown arrows). Inset: Higher power magnification of Ki-67+ stromal cells. Scale bars: 75 μm (B–D). Original magnification: ×400 (B–D, insets). H&E, hematoxylin and eosin; IHC, immunohistochemical; OTC, organotypic tissue culture; PBMC, peripheral blood mononuclear cell.