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. 2018 May 23;12:336. doi: 10.3389/fnins.2018.00336

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Copyright © 2018 Adami, Pagano, Colombo, Platonova, Recchia, Chiaramonte, Bottinelli, Canepari and Bottai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth curve and doubling time. (A) Proliferation capabilities comparison between CTR and HU samples. Each node of the curve represents a passage. The analysis was performed for 60 days of growth. CTR1; CTR2, CTR3; HU1; HU2; HU3. Dashed lines represent the average of CTR (black) and HU (gray). The average curves were built as the average of the slope of the CTR curves and HU curves with the constraint that the curve has value 250,000 at day 0. (B) Doubling time of proliferation expressed in days was calculated as the average of the doubling time of the single samples by means of non-linear fit regression. Black CTR, Gray HU. ^**p < 0.0089.

Inline graphic — Growth curve and doubling time. (A) Proliferation capabilities comparison between CTR and HU samples. Each node of the curve represents a passage. The analysis was performed for 60 days of growth. CTR1; CTR2, CTR3; HU1; HU2; HU3. Dashed lines represent the average of CTR (black) and HU (gray). The average curves were built as the average of the slope of the CTR curves and HU curves with the constraint that the curve has value 250,000 at day 0. (B) Doubling time of proliferation expressed in days was calculated as the average of the doubling time of the single samples by means of non-linear fit regression. Black CTR, Gray HU. ^**p < 0.0089.