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. 2018 May 23;12:336. doi: 10.3389/fnins.2018.00336

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Copyright © 2018 Adami, Pagano, Colombo, Platonova, Recchia, Chiaramonte, Bottinelli, Canepari and Bottai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunostaining of the differentiated cells. β-tubulin III (red, Alexa 555 Goat anti-mouse antibody) and GFAP (green, Alexa 488 Goat anti-rabbit antibody), nuclei (blue, DAPI) staining. (A,C) Bar 50 μm. (B,D) higher magnification, Bar 20 μm. (E) Quantitative analysis of β-tubulin III marker expression in CTR (n = 4) and HU (n = 4), ^*p < 0.05. O4 (red, Alexa 555 Goat anti-mouse antibody), nuclei (blue, DAPI) staining (F) CTR, (G) HU. (H) Quantitative analysis of O4 marker expression in CTR (n = 4) and HU (n = 4), ^*p < 0.05. Bars 20 μm.