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. 2018 May 28;17:1533034618775509. doi: 10.1177/1533034618775509

Figure 2.

Figure 2.

Rho-associated coiled coil-containing protein kinase 1 is a direct target of miR-199a in SW480 cells. A, Sequence alignment of miR-199a and the ROCK1 3′-UTR, which contains one predicted miR-199a binding site. The seed-recognizing sites in the ROCK1 3′-UTR are indicated in red, while the sites in the mutant ROCK1 3′-UTR are indicated in blue. B, Luciferase assay in SW480 cells cotransfected with miR-199a and a luciferase reporter containing the ROCK1 3′-UTR (WT) or a mutant (Mut). C, The miR-199a transfection affects ROCK1 mRNA levels. D, The miR-199a transfection affects ROCK1 protein levels. *P < .05. mRNA indicates messenger RNA; miR-199a, microRNA-199a; ROCK1, Rho-associated coiled coil-containing protein kinase 1; 3-UTR, 3-untranslated region; WT, wild type.