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Total RNA was extracted at 24 h after OMT (0, 6, 12, 18 mmol/L) treatment. The mRNA levels of GRP78/Bip (A), CHOP (B), caspase-4 (C), PERK (D), IREI (E) and ATF6 (F) were determined by RT-qPCR. GAPDH was used as a loading control, Results are expressed as 2−ddCt values. *P < 0.05, **P < 0.01 vs the control group.
