Table 3.

Fold changes in the expression of eight genes associated with biofilm formation.

				Fold change relative to controla,b
Category	Gene symbol	Gene description	Function	DD2	ATCC 10012	JCM 1022	ATCC 7469
Carbohydrate metabolism-promoting genes	ftf	Fructosyltransferase	Synthesis of fructan polymers from sucrose	0.29*	1.66	0.53	0.67
	gtfB	Glucosyltransferase B	Synthesis of adhesive extracellular glucans from sucrose	0.73	1.81	0.74	0.51
	gtfC	Glucosyltransferase C	Synthesis of adhesive extracellular glucans	0.64	1.60	0.81	0.90
Regulatory protein-encoding genes	brpA	Biofilm regulatory protein A	Response to environmental stress and biofilm development – putative surface-associated polypeptide	0.41*	1.87	1.65	1.39
	comDE	Competence-stimulating peptide	Regulation of genetic transformation and biofilm formation	0.21*	1.78	1.54	0.57
	vicR	Histidine kinase two-component regulatory system	Modulation of adherence and biofilm formation, and regulation of the expression of virulence-associated genes	0.23*	1.03	0.78	0.13*
Adhesion-promoting genes	gbpB	Glucan-binding protein	Adhesion to glucans and promotion of plaque formation	0.30*	0.67	0.44*	0.29*
	spaP	Cell wall-associated adhesin P1 or multi-functional adhesin	Sucrose-independent adhesion	0.14*	0.52	0.36*	0.28*

DD2, supplemented with DD2 culture supernatant; 10,012, supplemented with ATCC 10,012 culture supernatant; 3141, supplemented with JCM 1022 culture supernatant; 7469, supplemented with ATCC 7469 culture supernatant.

^aControl, Streptococcus mutans cultured in unsupplemented NB for 24 h.

^bFold change ≥1.5 or ≤0.5 was considered statistically significant, indicated by *.