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. 2018 May 23;6(10):e13706. doi: 10.14814/phy2.13706

Figure 5.

Figure 5

Satellite cells harvested from neonatal piglets treated with a control milk replacer diet (n = 10), or a control diet supplemented with 0.25% (T 0.25, n = 10) or 0.5% (T 0.5, n = 9) tributyrin for 21 days were induced to differentiate for 48 h. (A) Representative images of immunofluorescently stained myotubes with anti‐MyHC (green). Nuclei were visualized with DAPI and counted using Zeiss ZEN Pro automated image analysis. (B) Bar graph depicting the fusion percentages (≥2 nuclei expressing MyHC/total nuclei) in satellite cells from those animals treated with either control diet, or a control diet supplemented with 0.25% (T 0.25) or 0.5% (T 0.5) tributyrin. Bars not sharing superscripts differ significantly, P < 0.05.