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. 2018 May 23;9:1114. doi: 10.3389/fimmu.2018.01114

Figure 3.

Figure 3

ProteOn based affinity array of R11-NLS-pep8 toward proliferating cell nuclear antigen (PCNA) and cell viability screening of human and murine cell lines. (A,B) ProteOn array result showing the binding of human PCNA at protein concentration range of; 0 nM (X base-line), 15.6 nM (yellow), 31.2 (purple), 62.5 nM (green), 125 nM (blue), 250 nM (red), to bond R11-NLS-pep8 and R11-NLS-pep8short, respectively. Murine B16 melanoma, human PANC-1 (pancreas ductal adenocarcinoma), HepG2 (liver hepatocellular carcinoma), and MDA-MB-231 (breast adenocarcinoma) cancer cell lines are used for checking the effect of R11-NLS-pep8 and R11-NLS-pep8short on cell viability in a PrestoBlue assay. The results are shown in respect to the basal viability indicated using equivalent % DMSO (background). ED50 was calculated using a sigmoidal dose–response curve. ED50 values were 7.1, 7.8, 5.9, and 5.6 µM, respectively, for B16, PANC-1, HepG2, and MDA-MB-231 (C–F).