Table 2.

Primer Sequences for SPM Receptors and Lipoxygenase Enzymes

Primer	Sequence (Forward, Reverse)
β-actin	5′-TCCGATGCCCTGAGGCTCTTTTC-3′
	5′-CTTGCTGATCCACATCTGCTGGAA-3′
GAPDH	5′-GGTGTGAACGGATTTGGCCGTATT-3′
	5′-GTCGTTGATGGCAACAATCTCCAC-3′
BLT1	5′-TGACTTAGCTGACAGACCTAGA-3′
	5′-AGGATGCTCCACACTACAAAG-3′
ChemR23	5′-CCACTACCAGAACAACTGAGAA-3′
	5′-CTCCTCCAAGTCCACAAAGTAG-3′
ALX/FPR2	5′-CACAGGAACCGAAGAGTGTAAG-3′
	5′-GGAATCCAGCTACCCAAATCA-3′
Alox5	5′-CTGCTGGACAAGGCATTCTA-3′
	5′-TCCATCCCTCAGGACAATCT-3′
12/15lox	5′-CTCTCAAGGCCTGTTCAGGA-3′
	5′'-GTCCATTGTCCCCAGAACCT-3′

Primer sequences custom made from Invitrogen for analysis of SPM receptors, LOX enzymes, and housekeeping genes for whole lung homogenate and quantitative RT-PCR.