Figure 1.

Experimental design and average values for maternal and litter parameters. A, The mixture of 23 chemicals added, in equimass proportions, to the drinking water. B, Pregnant C57Bl/6 dams were placed on control water (0.2% ethanol) or water containing 0.1 or 1 μg/ml of the mixture on day of pregnancy (GD0), through weaning (PND21). There were 10 dams in each group. At maturity (6–8 weeks), offspring of separate dams from each treatment group were randomly assigned to one of four assessment groups for the indicated assessments: immune organ cellularity, HDM-induced airway disease, IAV infection, or EAE. Within each immunological assessment, there were at least 8 nonsibling males and 8 nonsibling females from each developmental exposure group. C, Average daily water consumption by dams. D, Average number of male and female offspring per litter according to treatment group. E, F, Average body weight of adult (8-week) female and male offspring by exposure group. Error bars represent SEM.