Fig. 1.

NGS can be used to identify the karyotype of mouse cells of known ploidy. A) Sequencing results are shown of genomic DNA isolated from pooled mouse embryonic fibroblasts engineered to have stable Trisomy 16 (copy number of 3). This cell line is of female origin. B) Average yield of DNA isolated from individual fibroblasts, polar bodies, oocytes, or blastocysts following WGA. Representative sequencing plots are shown from a single Trisomy 16 female MEF (C) and a single euploid male MEF (D) following WGA and NGS. *Autosomes that have copy numbers that deviate from 2, which indicates aneuploidy.