Table 2.
Summary of statistical tests conducted on the microbiota samples collected from the gut of channel Ictalurus punctatus and blue catfish I. furcatus and their environment (water and diet).
| Parameter | Statistical test | Index | Main factor P-value | Nested factor P-value |
|---|---|---|---|---|
| Alpha-diversity (Gut vs. Environ.) | Wilcoxon rank-sum testA | Observed | 0.004∗ | |
| Chao1 | 0.007∗ | |||
| Alpha-diversity | Nested ANOVA | Observed | 0.947 | 0.108 |
| Chao1 | 0.939 | 0.068 | ||
| Beta-diversity | PERMDISP2 | UniFrac | 0.981 | |
| wUniFrac | 0.040∗ | |||
| Nested PERMANOVA | UniFrac | 0.185 | 0.875 | |
| wUniFrac | 0.211 | 0.095 | ||
| Beta-diversity vs. host genetic distance (Rst) | Mantel test | UniFrac | 0.806 | |
| wUniFrac | 0.333 | |||
AStatistical test conducted between sample type (gut-associated vs. environment-associated), all other tests conducted using only gut-associated samples tested by the grouping factors of fish species (main) and fish strain (nested). ∗Indicates a rejection of the null hypothesis of no difference among experimental groups (P ≤ 0.05).